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I'm only up to hydroponics so far, but my vote goes for the noise that plays shortly after you put the toxin canister in the... thing.

Especially lovely if you have the volume cranked up.
you apparently haven't tried to hit a ripped off head with your wrench yet.
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voodoo47: you apparently haven't tried to hit a ripped off head with your wrench yet.
/thread

Pretty much any sounds associated with the cyborg midwives chill me.