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Has to be the line from The Witcher 2 where Philippa kisses helpless Saskia during some magic stuff.

"My favorite type of magic - lesbomancy..."
the best games for one liners RESIDENT EVIL

resident evil 1

Barry: That was too close! You were almost a Jill sandwich!

resident evil 2

Leon: It’s over

Claire: No. I have to find my brother.

Leon: You’re right. This is just the beginning.

ahh i had to look for the opening in resident evil 1. the best opening ever hahaha, the remake doesn´t do it justice

https://www.youtube.com/watch?v=YUtaxyA6W2o
"YOU CAN'T CUT BACK ON FUNDING!! YOU WILL REGRET THIS!!!!"

-Transit adviser guy from SimCity 2000 SE (if you try to lower the funding for transit)
Plunder bunny!

-- The Secret of Monkey Island
"I'm gonna rob you of your organs, taffer boy!"

-Downwind guard from Thief GOLD

"Now everyone will know that I'm a master thief... waltzing right into a bank vault."

-Garrett, Thief II (after breaking into the vault at First City Bank & Trust)
"Exterminate! Exterminate!"

-Joey, Beneath a Steel Sky
'Leave me alone!' - the human peasants in Warcraft II. Actually, all of the laborer quotes from that game are hilarious. Every time you'd click the workers they seemed to get more and more annoyed.
Monkey Island:
"How appropriate you fight like a cow."
"Never pay more than 20 dollars for a computer game."

Metal Gear (nes)
"The truck have started to move."

Lastly, Ive never seen this from playing ,but have read about it.
"Yo gangsta! Get ready to gang bang!" Bust-a-Groove
Post edited April 02, 2015 by lazylazyjoe
"chicken chaser, he chases chickens!" -Fable

" Oh fuck it, I don't even want to be here" -random bad guy in Tomb Raider

And Skyrim's Cicero is full of fun, but this one had my dying when I first heard it,
"Cicero's heard about the Butcher, interesting knife work. Just... Stab, stab, stab, stab, stab! And then... Stab, stab, stab, stab, stab!"
"I'm not big into books."

JC Denton says it a few times in Deus EX.
Just about every one-line that Duke Nukem had in Manhatten Project.
Post edited April 02, 2015 by austen1000
"He wasted the whole squad!"
F.E.A.R.

"No fucking way!"
F.E.A.R.. answer of replica soldier when his leader wants him to attack me after I wasted a whole squad!
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NowaAnglia: "Bang! You're dead, Max Payne." - Vladimir Lem, pointing his finger gun at Max Payne in Max Payne 2.
Vlad in GTA 4 also references this line when he tell's Niko, "Bang Bang, you are dead!" Makes that a nice one liner in its own right!
"Then you may die knowing the truth"
Kain, Blood Omen 2
"Tough, don't blame us. Blame yourself or God" - Delita, Final Fantasy Tactics

That was some cold stuff to someone just before you kill them